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LS 6000 Scintillation System User’s Manual
PN 247971-F
7-6
How Sample Preparation Affects Results
Distinguishing Sample And Instrument Variability
1. The standards and your samples both meet the above criteria. This means the instrument
is working. There is still the possibility of an intermittent problem that did not arise over
this test period. Further long term study is required.
2. The standards check out but your samples vary: This means the instrument is working
and your sample preparation procedures must be reviewed.
3. The standards don’t meet the criteria: Regardless of how your samples perform, if the
standards do not meet the criteria outlined within some small limits, then the instrument
is not performing properly. In this case, a Authorized Beckman Service Representative
should be contacted.
3
H Counting Efficiency
For
3
H it is necessary to correct for the radioactive decay since the samples were prepared.
This can be done by using the half life table (Appendix D). The
3
H standard will have the date
of calibration and the dpm on that date printed on the vial. Calculate t (current date minus
date of calibration, in years). Divide t by the half life T (12.35 years
3
H). Find this value in the
t/T column of the half life table. Use the corresponding fraction remaining value to multiply
the standard DPM. The result is the DPM remaining on the selected date.
Use the CPM for Isotope 1 for the ~ standard, and the CPM for Isotope 1 for the background
sample. The background for this calculation is insignificant compared to the CPM of the
standard but is given for completeness. Calculate the ~ efficiency from the equation:
14
C Counting Efficiency
For the
14
C efficiency, use the
14
C CPM for Isotope 2, Wide. Calculate the efficiency as
described for
3
H.
Acceptable values meet these system specifications:
If any results fall to meet these values, check the following:
1. Inspect the sample vials to ensure they are securely sealed.
2. Ensure the instrument is calibrated and set up properly.
3. Verify that you have accurately corrected for decay using the Half-Life Tables, and that
your computations are correct.
If it appears that system efficiency is less than the specifications, contact your Authorized
Beckman Service Representative.
% Efficiency
CPM - Background x 100
DPM
=
3
H: 59% in wide open window≥
14
C: 95%≥
H#: 0 5±
- LS 6000 Series 1
- Table of Contents 3
- Introduction 11
- Principles of Operation 12
- Operating the Instrument 12
- Operating Modes 13
- 1.3 Specifications 14
- Precautions and Limitations 15
- Figure 1.1 Standard Vial 15
- Miniature Vials 16
- 1.5 Hazards 17
- Maintenance 18
- Getting Started 19
- 2.2 Power Failure 21
- 2.3 Operating Controls 21
- Operator Control Key pad 22
- Using the Operating Controls 24
- 2.4 Using the Racks 25
- Using the Racks 26
- Setting Up the Racks 27
- 2.5 Preparing the Printer 29
- 2.6 System Setup 30
- Energy Scale 31
- Printer Paging 32
- Allow Interrupt Count 32
- System Setup 33
- Figure 2.9 RS232 Setup Menu 33
- Date/Time 35
- Auto DPM 35
- 2.7 Calibration 36
- Counting Samples 39
- Starting the Automatic Count 40
- Conducting Count Single Rack 41
- 3.4 Auto DPM 46
- 3.5 Multi-Task 47
- Interrupt Count in Multi-Task 48
- Multi-Task 50
- Interrupt Data 51
- Setting Up User Programs 59
- 4.2 Editing A User Program 61
- Editing A User Program 62
- Comments 65
- Counting Time 65
- Liquid or Xtal Scintillator 65
- Isotope 1 65
- Isotope 2 and Isotope 3 66
- Edit Other Parameters 67
- Counting Precision 69
- Background/Blank Subtraction 69
- Lum-Ex Correction 71
- Phase Monitor 72
- Low Level 72
- Low Count Reject 72
- Output Formats 72
- Count Data 75
- Raw Data 75
- Collecting Spectral Data 76
- 4.3 Copy User Program 77
- Copy User Program 78
- 5.2 CPM/Xtal CPM 80
- CPM/Xtal CPM 81
- Results for CPM/Xtal CPM 83
- 5.4 Auto DPM 85
- Setting Up Auto DPM 86
- Loading Samples for Auto DPM 86
- Results for Auto DPM 86
- Setting Up DPM 87
- 5.6 Xtal DPM 89
- Xtal DPM 90
- Setting Up Xtal DPM 91
- Loading Samples for Xtal DPM 92
- 5.7 DPM % of Reference 93
- Setting Up DPM % of Reference 94
- Xtal SL DPM % of Reference 95
- 5.9 Single Photon Monitoring 98
- Data Calculation 100
- Single Photon Monitoring 100
- Isotope/DPM Libraries 103
- Accessing the Isotope Library 104
- Printing the Isotope Library 104
- The Isotope Library 105
- Setup Menu 106
- PN 247971-F 108
- 5 minutes 109
- 6.2 Setting Up A Quench Curve 110
- Setting Up A Quench Curve 112
- Counting the Standards 114
- Editing Quench Curves 120
- Deleting A Quench Curve 124
- Manual Entry of Quench Curves 125
- 7.1 Chemiluminescence 127
- Interpretation of Results 128
- Reducing Single Photon Events 128
- 7.3 Two Phase Samples 129
- Procedures 131
- Analysis of Data 131
- H Counting Efficiency 132
- C Counting Efficiency 132
- Background 133
- Contamination 133
- Instrument Specifications 135
- Temperature Control Accessory 138
- Installation Requirements 139
- Electrical Requirements 140
- 0.5 to 1.0 m in 140
- Environment Requirements 142
- . Note that the output 144
- Datum IDs 146
- CHAPTER 1, PART 31 167
- Region I 170
- Region II 170
- Region III 170
- Region IV 170
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