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LS 6000 Scintillation System User’s Manual
PN 247971-F
3-2
Counting Samples
Conducting An Automatic Count
You can load up to 28 standard vial racks or 36 miniature racks or Bio-Vial racks. With
Versa-Rack installed, standard, miniature and Bio-Vial racks may be intermixed within the
same run. Care must be taken not to place more racks in the sample changer than it is capable
of using. An error message is displayed if the sample changer is overloaded.
If you want to calibrate the instrument before counting your samples, first place the Calibrate
Rack into the sample changer. Be sure this rack has the Auto Calibrate Card installed and the
14
C unquenched standard is in position #1. Refer to Section 2.7 for more information on
calibration.
NOTE Programs using blanks, replicates, % of Reference, or Xtal DPM require specific sample loading
sequences within the racks. Refer to the appropriate sections in Section 5 for more information on the
required loading sequences of the samples within the racks.
Load your samples that have been placed in the appropriate Sample Racks. The first Sample
Rack must be the rack with the User Number Card installed that corresponds to the User
Program you wish to use. During Automatic Count, only the first rack of samples requires a
User Number Card. All following racks are counted under the same program, until a new
User Number Card or Halt Card is encountered.
Sample Racks may be loaded even if they are less than full. The instrument skips past the
empty positions and proceeds to the next rack. Rack Number Cards are not required on the
racks. If they are installed, however, Sample Racks can be used in any rack number order. The
system reads and prints the number from the card before processing the rack.
Place all the racks together that are to be counted under the same User Program. If one or
more additional sample sets are to be counted under different User Numbers during the same
Automatic Count, make certain that the first rack in each set bears the correct User Number
Card. When a Sample Rack with a User Number Card reaches the position for processing, the
instrument “reads” the new user number and changes the processing parameters to those
specified in that User Program.
When all the Sample Racks to be counted are loaded, place the red Halt Rack behind the last
rack of samples to terminate Automatic Count.
NOTE Samples placed in the Halt Rack are not counted. Do not place samples to be counted in the Halt
Rack.
Additional sample sets may be loaded after Automatic Count has been initiated. Load the new
sample sets in front of the Halt Rack, being careful that the new sample sets are not loaded in
the middle of a set of samples waiting to be counted.
Starting the Automatic Count
NOTE Check that the printer is ON (it should be left on). Also check that the ON LINE light is lit, and that
paper is loaded and is at the top of the form. Refer to Section 2.5 for more information on setting up the
printer.
Automatic Count is initiated from the Main Menu. Highlight Automatic Counting and press
SELECT to begin the counting process. The Main Editing Window displays instructions to
load your samples. Load the samples as described in Section and press START. The racks are
advanced to the rear of the instrument and counting commences. A typical screen displayed
during Automatic Count is shown in Figure 3.2.
- LS 6000 Series 1
- Table of Contents 3
- Introduction 11
- Principles of Operation 12
- Operating the Instrument 12
- Operating Modes 13
- 1.3 Specifications 14
- Precautions and Limitations 15
- Figure 1.1 Standard Vial 15
- Miniature Vials 16
- 1.5 Hazards 17
- Maintenance 18
- Getting Started 19
- 2.2 Power Failure 21
- 2.3 Operating Controls 21
- Operator Control Key pad 22
- Using the Operating Controls 24
- 2.4 Using the Racks 25
- Using the Racks 26
- Setting Up the Racks 27
- 2.5 Preparing the Printer 29
- 2.6 System Setup 30
- Energy Scale 31
- Printer Paging 32
- Allow Interrupt Count 32
- System Setup 33
- Figure 2.9 RS232 Setup Menu 33
- Date/Time 35
- Auto DPM 35
- 2.7 Calibration 36
- Counting Samples 39
- Starting the Automatic Count 40
- Conducting Count Single Rack 41
- 3.4 Auto DPM 46
- 3.5 Multi-Task 47
- Interrupt Count in Multi-Task 48
- Multi-Task 50
- Interrupt Data 51
- Setting Up User Programs 59
- 4.2 Editing A User Program 61
- Editing A User Program 62
- Comments 65
- Counting Time 65
- Liquid or Xtal Scintillator 65
- Isotope 1 65
- Isotope 2 and Isotope 3 66
- Edit Other Parameters 67
- Counting Precision 69
- Background/Blank Subtraction 69
- Lum-Ex Correction 71
- Phase Monitor 72
- Low Level 72
- Low Count Reject 72
- Output Formats 72
- Count Data 75
- Raw Data 75
- Collecting Spectral Data 76
- 4.3 Copy User Program 77
- Copy User Program 78
- 5.2 CPM/Xtal CPM 80
- CPM/Xtal CPM 81
- Results for CPM/Xtal CPM 83
- 5.4 Auto DPM 85
- Setting Up Auto DPM 86
- Loading Samples for Auto DPM 86
- Results for Auto DPM 86
- Setting Up DPM 87
- 5.6 Xtal DPM 89
- Xtal DPM 90
- Setting Up Xtal DPM 91
- Loading Samples for Xtal DPM 92
- 5.7 DPM % of Reference 93
- Setting Up DPM % of Reference 94
- Xtal SL DPM % of Reference 95
- 5.9 Single Photon Monitoring 98
- Data Calculation 100
- Single Photon Monitoring 100
- Isotope/DPM Libraries 103
- Accessing the Isotope Library 104
- Printing the Isotope Library 104
- The Isotope Library 105
- Setup Menu 106
- PN 247971-F 108
- 5 minutes 109
- 6.2 Setting Up A Quench Curve 110
- Setting Up A Quench Curve 112
- Counting the Standards 114
- Editing Quench Curves 120
- Deleting A Quench Curve 124
- Manual Entry of Quench Curves 125
- 7.1 Chemiluminescence 127
- Interpretation of Results 128
- Reducing Single Photon Events 128
- 7.3 Two Phase Samples 129
- Procedures 131
- Analysis of Data 131
- H Counting Efficiency 132
- C Counting Efficiency 132
- Background 133
- Contamination 133
- Instrument Specifications 135
- Temperature Control Accessory 138
- Installation Requirements 139
- Electrical Requirements 140
- 0.5 to 1.0 m in 140
- Environment Requirements 142
- . Note that the output 144
- Datum IDs 146
- CHAPTER 1, PART 31 167
- Region I 170
- Region II 170
- Region III 170
- Region IV 170
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