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LS 6000 Scintillation System User’s Manual
PN 247971-F
4-12
Setting Up User Programs
Editing A User Program
Blanks are samples that are counted at the beginning of your unknown samples, and the value
of the blanks are subtracted from each of the subsequent samples. Blanks are counted along
with the samples, and have the same counting time and counting precision as the samples.
The counting precision obtained when the blank is counted is used to correct the precision of
the unknown samples.
To change the settings, highlight “Background /Blank Subtraction” and press SELECT. The
Main Editing Window displays prompts to count the blanks and to enter values for
Back-ground. Blanks and/or backgrounds may be used.
To change the setting for blanks, highlight “Count Blanks” and choose Yes or No. To change
the background values, highlight the background for each isotope and enter the cpm and
counting precision from a previously counted background sample. The counting precision
must be entered as a 2 sigma value. Press PREVIOUS MENU to return to the Review/Edit
Menu shown in Figure 4.4.
NOTE Blank vial(s), if selected, must be loaded in the first position of the first rack, followed by an empty
space. Refer to Section 5 for loading sequences using blanks.
Quench
Quench reduces the light output from the sample and hence affects the accuracy of the cpm.
Quench may result from color in the sample or from chemicals that affect energy transfer in
the cocktail. The instrument provides a Quench Monitor, which determines the extent of
quench in the sample and prints out a number which is proportional to the amount of
quench. Two methods of monitoring quench are available; IC# and H# Plus (if installed).
When H# Plus is installed, Automatic Quench Compensation (AQC), a method of
automatically adjusting the window settings as a function of quench, is available. This
adjustment in window settings, based on the value of H#, is performed to keep the spill of the
- LS 6000 Series 1
- Table of Contents 3
- Introduction 11
- Principles of Operation 12
- Operating the Instrument 12
- Operating Modes 13
- 1.3 Specifications 14
- Precautions and Limitations 15
- Figure 1.1 Standard Vial 15
- Miniature Vials 16
- 1.5 Hazards 17
- Maintenance 18
- Getting Started 19
- 2.2 Power Failure 21
- 2.3 Operating Controls 21
- Operator Control Key pad 22
- Using the Operating Controls 24
- 2.4 Using the Racks 25
- Using the Racks 26
- Setting Up the Racks 27
- 2.5 Preparing the Printer 29
- 2.6 System Setup 30
- Energy Scale 31
- Printer Paging 32
- Allow Interrupt Count 32
- System Setup 33
- Figure 2.9 RS232 Setup Menu 33
- Date/Time 35
- Auto DPM 35
- 2.7 Calibration 36
- Counting Samples 39
- Starting the Automatic Count 40
- Conducting Count Single Rack 41
- 3.4 Auto DPM 46
- 3.5 Multi-Task 47
- Interrupt Count in Multi-Task 48
- Multi-Task 50
- Interrupt Data 51
- Setting Up User Programs 59
- 4.2 Editing A User Program 61
- Editing A User Program 62
- Comments 65
- Counting Time 65
- Liquid or Xtal Scintillator 65
- Isotope 1 65
- Isotope 2 and Isotope 3 66
- Edit Other Parameters 67
- Counting Precision 69
- Background/Blank Subtraction 69
- Lum-Ex Correction 71
- Phase Monitor 72
- Low Level 72
- Low Count Reject 72
- Output Formats 72
- Count Data 75
- Raw Data 75
- Collecting Spectral Data 76
- 4.3 Copy User Program 77
- Copy User Program 78
- 5.2 CPM/Xtal CPM 80
- CPM/Xtal CPM 81
- Results for CPM/Xtal CPM 83
- 5.4 Auto DPM 85
- Setting Up Auto DPM 86
- Loading Samples for Auto DPM 86
- Results for Auto DPM 86
- Setting Up DPM 87
- 5.6 Xtal DPM 89
- Xtal DPM 90
- Setting Up Xtal DPM 91
- Loading Samples for Xtal DPM 92
- 5.7 DPM % of Reference 93
- Setting Up DPM % of Reference 94
- Xtal SL DPM % of Reference 95
- 5.9 Single Photon Monitoring 98
- Data Calculation 100
- Single Photon Monitoring 100
- Isotope/DPM Libraries 103
- Accessing the Isotope Library 104
- Printing the Isotope Library 104
- The Isotope Library 105
- Setup Menu 106
- PN 247971-F 108
- 5 minutes 109
- 6.2 Setting Up A Quench Curve 110
- Setting Up A Quench Curve 112
- Counting the Standards 114
- Editing Quench Curves 120
- Deleting A Quench Curve 124
- Manual Entry of Quench Curves 125
- 7.1 Chemiluminescence 127
- Interpretation of Results 128
- Reducing Single Photon Events 128
- 7.3 Two Phase Samples 129
- Procedures 131
- Analysis of Data 131
- H Counting Efficiency 132
- C Counting Efficiency 132
- Background 133
- Contamination 133
- Instrument Specifications 135
- Temperature Control Accessory 138
- Installation Requirements 139
- Electrical Requirements 140
- 0.5 to 1.0 m in 140
- Environment Requirements 142
- . Note that the output 144
- Datum IDs 146
- CHAPTER 1, PART 31 167
- Region I 170
- Region II 170
- Region III 170
- Region IV 170
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