Motorola LS6000B-U Guía de usuario descargar pdf (Pagina 81)

LS 6000 Scintillation System User’s Manual

PN 247971-F

5-3

Data Calculation

CPM/Xtal CPM

Half-Life Correction

Sample data can be half-life corrected to the standardization date of the isotopes used in the

samples. This date is printed by the manufacturer on the label of the isotope container. If a

date is not entered (a zero is entered), then half-life correction is calculated from the start of

the count cycle. A half-life for the isotope must be stored in Isotope Library to obtain half-life

correction. For accurate half-life correction, the real time clock in the instrument must be set

to the correct date and time. Refer to Auto DPM Section 2 for setting the clock.

To enter the half-life correction date of an isotope, highlight “Half Life Date” for that isotope

and enter the required date in this format: dd mmm yyyy hr:min(2 digits for day, a 3 letter

code for month, and 4 digits for the year, 2 digits for the hour and 2 digits for the minutes).

NOTE When a CPM/Xtal CPM or SL CPM % of Reference/Xtal SL CPM % of Reference program is selected,

half-life correction is only performed for single label samples. When Wide or Manual is selected as an

isotope, half-life correction is not performed on the data from that window.

Count Sample

This feature provides repeated counts of each sample, and is useful to detect static problems,

two-phase samples, sample precipitation or any time-dependent sample variations. Refer to

Section 7 for more information on how these sample preparation problems affect the results.

When a value greater than one is entered, the printout shows the results of each sample

count, the average, and the coefficient of variation (CV).

To change the number of times to count the sample, highlight “Count Sample” and enter the

desired number of times to count the sample.

Replicates

This feature makes it possible to run experimental samples in replicate to detect experimental

variation and to average out pipetting variations. For example, experiments may be set up in

duplicate (two samples per data point), triplicate (three samples per data point), etc.

The printout for replicate counts includes individual counts, the average for the vials in each

replicate group, and the coefficient of variation (CV). Sample repeats and replicates can be

used together.

To change the number of replicates, highlight “Replicates” and enter the number of sample

replicates.

Count Sample Set

The entire set of samples can be recounted a number of times. When another User Number

Card or Halt Card is detected, the sample changer backs up to the beginning of the sample set

and recounts the samples. This is repeated until the entry for Count Sample Set is satisfied.

To change the number of times to count the sample set, highlight “Count Sample Set’ and

enter the desired number.

Factor

This feature enables you to obtain a printout in which the final results (cpm, dpm, or % of

Reference values) have been multi-plied by a constant:

1 2 ... 76 77 78 79 80 81 82 83 84 85 86 ... 179 180

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Motorola LS6000B-U Guía de usuario Pagina 81

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