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LS 6000 Scintillation System User’s Manual
PN 247971-F
2-19
Getting Started
Temperature Control Accessory
2
NOTE The unquenched
14
C standard must be the first sample in the Calibrate Rack. If a vial is
detected in position #2 of the Calibrate Rack, Auto DPM Calibration is also per-formed. Refer to
Section 3 Auto DPM Calibration for information on Auto DPM Calibration. Other vials in this rack
are not counted. If calibrating before an Automatic Count, samples for counting must be loaded in
the Sample Racks following the Calibrate Rack, not in the Calibrate Rack.
2. Immediately following the Calibrate Rack, place the Halt Rack (described in Section 2
Setting Up the Racks). If calibrating before Automatic Count, place the sample racks to
count immediately after the Calibrate Rack, followed by the Halt Rack.
3. Press START. The instrument counts the standard and makes the appropriate internal
corrections. A message is printed stating whether calibration was successful or not. If
calibration was unsuccessful, the instrument retains the last calibration.
When Calibration Should Be Done
The instrument should be calibrated daily for the first week or two after installation, until the
phototubes settle in.
After this initial warm-up period, calibration can be performed as follows:
r Once a month.
r Prior to initiating dpm programs (recommended).
r When setting up a new isotope automatically using the New Isotope Setup program
(required).
r Prior to setting up quench curves (required).
r Prior to initiating an Auto DPM program. Refer to Section 3 Auto DPM Calibration for
information on Auto DPM Calibration (recommended).
2.8 Temperature Control Accessory
Temperature Control Accessory, if installed, maintains the sample changer at a constant
temperature. Always operate your LS System with the changer base cover closed when the
Temperature Control Accessory is being used. For most efficient performance, limit the
frequency of opening the changer base cover. If additional samples are being placed on the
changer base, allow sufficient time for the new samples to cool down before counting these
samples.
Under high humidity, some condensation will
occur on the changer base, the air vents, the
changer base
cover, etc. This is normal. To minimize condensation, ensure that the cooling
unit and the IS unit are positioned as close together as possible and
their air vents are correctly
aligned.
Operating the Temperature Control Accessory beyond the operating specifications
will cause excessive condensation.
- LS 6000 Series 1
- Table of Contents 3
- Introduction 11
- Principles of Operation 12
- Operating the Instrument 12
- Operating Modes 13
- 1.3 Specifications 14
- Precautions and Limitations 15
- Figure 1.1 Standard Vial 15
- Miniature Vials 16
- 1.5 Hazards 17
- Maintenance 18
- Getting Started 19
- 2.2 Power Failure 21
- 2.3 Operating Controls 21
- Operator Control Key pad 22
- Using the Operating Controls 24
- 2.4 Using the Racks 25
- Using the Racks 26
- Setting Up the Racks 27
- 2.5 Preparing the Printer 29
- 2.6 System Setup 30
- Energy Scale 31
- Printer Paging 32
- Allow Interrupt Count 32
- System Setup 33
- Figure 2.9 RS232 Setup Menu 33
- Date/Time 35
- Auto DPM 35
- 2.7 Calibration 36
- Counting Samples 39
- Starting the Automatic Count 40
- Conducting Count Single Rack 41
- 3.4 Auto DPM 46
- 3.5 Multi-Task 47
- Interrupt Count in Multi-Task 48
- Multi-Task 50
- Interrupt Data 51
- Setting Up User Programs 59
- 4.2 Editing A User Program 61
- Editing A User Program 62
- Comments 65
- Counting Time 65
- Liquid or Xtal Scintillator 65
- Isotope 1 65
- Isotope 2 and Isotope 3 66
- Edit Other Parameters 67
- Counting Precision 69
- Background/Blank Subtraction 69
- Lum-Ex Correction 71
- Phase Monitor 72
- Low Level 72
- Low Count Reject 72
- Output Formats 72
- Count Data 75
- Raw Data 75
- Collecting Spectral Data 76
- 4.3 Copy User Program 77
- Copy User Program 78
- 5.2 CPM/Xtal CPM 80
- CPM/Xtal CPM 81
- Results for CPM/Xtal CPM 83
- 5.4 Auto DPM 85
- Setting Up Auto DPM 86
- Loading Samples for Auto DPM 86
- Results for Auto DPM 86
- Setting Up DPM 87
- 5.6 Xtal DPM 89
- Xtal DPM 90
- Setting Up Xtal DPM 91
- Loading Samples for Xtal DPM 92
- 5.7 DPM % of Reference 93
- Setting Up DPM % of Reference 94
- Xtal SL DPM % of Reference 95
- 5.9 Single Photon Monitoring 98
- Data Calculation 100
- Single Photon Monitoring 100
- Isotope/DPM Libraries 103
- Accessing the Isotope Library 104
- Printing the Isotope Library 104
- The Isotope Library 105
- Setup Menu 106
- PN 247971-F 108
- 5 minutes 109
- 6.2 Setting Up A Quench Curve 110
- Setting Up A Quench Curve 112
- Counting the Standards 114
- Editing Quench Curves 120
- Deleting A Quench Curve 124
- Manual Entry of Quench Curves 125
- 7.1 Chemiluminescence 127
- Interpretation of Results 128
- Reducing Single Photon Events 128
- 7.3 Two Phase Samples 129
- Procedures 131
- Analysis of Data 131
- H Counting Efficiency 132
- C Counting Efficiency 132
- Background 133
- Contamination 133
- Instrument Specifications 135
- Temperature Control Accessory 138
- Installation Requirements 139
- Electrical Requirements 140
- 0.5 to 1.0 m in 140
- Environment Requirements 142
- . Note that the output 144
- Datum IDs 146
- CHAPTER 1, PART 31 167
- Region I 170
- Region II 170
- Region III 170
- Region IV 170
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